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Test ID STICK Tick-Borne Antibodies, Modified 2-Tier, ELISA, Serum


Ordering Guidance


During the acute phase of an Anaplasma phagocytophilum, Ehrlichia chaffeensis, or Babesia infection, serologic tests are often nonreactive and detection by molecular assays is recommended. Polymerase chain reaction (PCR) testing is available to aid in the diagnosis of these cases; see EPCRB / Ehrlichia/Anaplasma, Molecular Detection, PCR, Blood and BABPB / Babesia species, Molecular Detection, PCR, Blood.



Specimen Required


Supplies: Sarstedt Aliquot Tube 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1.35 mL Serum

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Useful For

Diagnosis of Lyme disease and later stages of anaplasmosis, ehrlichiosis, and babesiosis

 

Sero-epidemiological surveys of the prevalence of the infection in certain populations

Profile Information

Test ID Reporting Name Available Separately Always Performed
ANAP Anaplasma phagocytophilum Ab, IgG,S Yes Yes
EHRC Ehrlichia Chaffeensis (HME) Ab, IgG Yes Yes
BABG Babesia microti IgG Ab, S Yes Yes
SLYME Lyme Ab Modified 2-Tier w/Reflex, S Yes Yes

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
TLYME Lyme IgM/IgG, WCS, EIA, S Yes No

Testing Algorithm

If the Lyme antibody result is positive or equivocal, then Lyme disease antibody confirmation will be performed at an additional charge.

 

For more information see Acute Tickborne Disease Testing Algorithm.

Method Name

ANAP, EHRC, BABG: Immunofluorescence Assay (IFA)

SLYME: Enzyme-Linked Immunosorbent Assay (ELISA)

Reporting Name

Tick-Borne Abs w/ Lyme MTTTA, S

Specimen Type

Serum

Specimen Minimum Volume

Serum: 1.1 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 10 days
  Frozen  14 days

Reference Values

ANAPLASMA PHAGOCYTOPHILUM ANTIBODY, IGG

<1:64

Reference values apply to all ages.

 

EHRLICHIA CHAFFEENSIS (HME) ANTIBODY, IGG

<1:64

Reference values apply to all ages.

 

BABESIA MICROTI IGG ANTIBODIES

<1:64

Reference values apply to all ages.

 

LYME ANTIBODY

Negative

Reference values apply to all ages.

Day(s) Performed

Monday through Friday

Report Available

2 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

See Individual Test IDs

CPT Code Information

86618

86666 x 2

86753

86617 x 2 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
STICK Tick-Borne Abs w/ Lyme MTTTA, S 103603-7

 

Result ID Test Result Name Result LOINC Value
81157 Anaplasma phagocytophilum Ab, IgG,S 23877-4
81128 Babesia microti IgG Ab, S 16117-4
81478 Ehrlichia Chaffeensis (HME) Ab, IgG 47405-6
SLYME Lyme Ab Modified 2-Tier w/Reflex, S 83081-0

NY State Approved

Yes

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.