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HelpTest ID MSTC1 Strict Criteria Sperm Morphology for Infertility Diagnosis and Treatment, Semen
Useful For
Diagnosing male infertility
Selecting the most cost-effective therapy for treating male-factor infertility
Quantifying the number of germinal and WBCs per mL of semen
Method Name
Kruger Criteria Strict Morphology
Reporting Name
Strict Sperm Morphology, SlideSpecimen Type
SemenOrdering Guidance
Additional Testing Requirements
Conventional semen analysis (FER / Semen Analysis, Semen) should be performed in conjunction with each strict criteria sperm morphology.
Shipping Instructions
Send specimen Monday through Friday
Necessary Information
Sperm count/mL is required (million/mL).
Specimen Required
Patient Preparation: Patient should have 2 to 7 days of sexual abstinence at the time of semen collection for accurate results.
Container/Tube: Slides
Specimen Volume: 2 slides-10 microL of liquefied semen on each slide
Collection Instructions:
1. If sperm concentration is <10 million/mL, centrifuge the specimen at 300 x G for 10 minutes before making slides.
2 Label 2 frosted slides in pencil with the patient's first and last name and the date of specimen collection. No adhesive labels.
3. Allow the semen to liquefy for 30 minutes.
4. Place 10 mcL of liquefied semen on the label end of each slide, and evenly smear the specimen using a plain slide (this process is the same as making a blood smear).
5. Allow the smears to air dry for 15 minutes before placing both slides into 1 slide holder for shipment.
Specimen Minimum Volume
A minimum count of sperm is needed; lab will determine
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Semen | Ambient | ||
Reference Values
Normal forms: ≥4.0%
Germinal cells/mL
<4 x 10(6) (normal)
≥4 x 10(6) (elevated germinal cells in semen are of unknown clinical significance)
WBC/mL
<1 x 10(6) (normal)
≥1 x 10(6) (elevated white blood cells in semen are of questionable clinical significance)
Day(s) Performed
Monday through Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
89398
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| MSTC1 | Strict Sperm Morphology, Slide | 48812-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| OVAL2 | Strict Morph NL | 10622-9 |
| ACRSM | Acrosom Defect | 66494-6 |
| HDSAB | Head Shape Abnormal | 66495-3 |
| HDZAB | Head Size Abnormal | 66496-1 |
| MD | Midpiece Defect | 10603-9 |
| TAILD | Tail Defect | 10604-7 |
| DBLF | Double Forms | 66497-9 |
| MULTI | Multiple Defects | 66498-7 |
| GERM3 | Germ Cells/mL | 10576-7 |
| WBC6 | WBC/mL | 10579-1 |
| CMT56 | Comment | 48767-8 |