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Test ID HK27M Histone H3 K27M Mutant (H3 K27M) Immunostain, Technical Component Only


Ordering Guidance


This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

 

Mayo Clinic Laboratories has multiple histone immunostains available. See table for ordering guidance. 

Test ID

Published name

Indication

Mayo Clinic slide label

HG34W

Histone 3.3 G34W (H3F3A G34W) Immunostain, Technical Component Only

Giant cell tumor of bone (GCTB)

H3 G34W

HK27M

Histone H3 K27M Mutant (H3 K27M) Immunostain, Technical Component Only

K27M mutant midline gliomas

H3 K27M

HISME

Histone H3 Trimethyl K27 (H3 K27me[3]) Immunostain, Technical Component Only

MPNST and K27M mutant midline gliomas

H3 K27me3

HK36M

Histone H3 K36M Mutant (H3F3 K36M) Immunostain, Technical Component Only

Chondroblastoma

H3 K36M


Shipping Instructions


Attach the green "Attention Pathology" address label (T498) and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.



Specimen Required


Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostain Technical Only Envelope

Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded tissue block


Useful For

Identifying the presence of altered H3 K27M protein

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name

Immunohistochemistry (IHC)

Reporting Name

Histone H3 K27M IHC, Tech Only

Specimen Type

TECHONLY

Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)
  Refrigerated 

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HK27M Histone H3 K27M IHC, Tech Only Order only;no result

 

Result ID Test Result Name Result LOINC Value
604990 Histone H3 K27M IHC, Tech Only Bill only; no result

NY State Approved

Yes

Forms

If not ordering electronically, complete, print, and send an Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.

Testing Algorithm

For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). For each additional technical component only IHC stain performed, an additional bill-only test ID will be reflexed and charged (IHTOA).