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Test ID HELIS Helicobacter pylori Culture with Antimicrobial Susceptibilities, Varies

Useful For

Recovery of Helicobacter pylori from gastric specimens for antimicrobial susceptibility testing of the organism (amoxicillin, clarithromycin, levofloxacin, metronidazole, rifampin, and tetracycline are routinely tested)

Method Name

Conventional Culture Technique with Minimal Inhibitory Concentration (MIC) (Agar Dilution or Broth Microdilution or Gradient Diffusion) or Disk Diffusion if appropriate

Reporting Name

Helicobacter pylori Culture + Susc

Specimen Type

Varies


Ordering Guidance


For test utilization options, see Helicobacter pylori Diagnostic Algorithm.



Shipping Instructions


Specimen must be received in laboratory within 48 hours of collection. Specimen should be collected and packaged as close to shipping time as possible.



Necessary Information


Specimen source is required; include the specific anatomic source.



Specimen Required


Preferred:

Specimen Type: Fresh tissue or biopsy

Sources: Gastric

Container/Tube: Sterile container

Specimen Volume: 0.5 x 0.2 x 0.2-cm sized piece of tissue

Collection Instructions: Acquire biopsied tissue; moisten with sterile saline

 

Acceptable:

Specimen Type: Fluid

Sources: Gastric brushings, gastric aspirate

Container/Tube: Sterile container

Specimen Volume: Entire collection or 0.5 mL

 

Specimen Type: Fresh tissue or biopsy

Sources: Duodenum

Container/Tube: Sterile container

Specimen Volume: 0.5 x 0.2 x 0.2-cm sized piece of tissue

Collection Instructions: Acquire biopsied tissue; moisten with sterile saline


Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated 48 hours

Reference Values

No growth of Helicobacter pylori

 

Susceptibility results are reported as minimal inhibitory concentration (MIC) in mcg/mL. Breakpoints (also known as clinical breakpoints) are used to categorize an organism as susceptible, susceptible-dose dependent, intermediate, resistant, or nonsusceptible according to breakpoint setting organizations, either the Clinical and Laboratory Standards Institute (CLSI) or the European Committee on Antimicrobial Susceptibility Testing (EUCAST), as applicable.

 

In some instances, an interpretive category cannot be provided based on available data; therefore, the following comment will be included on the report: There are no established interpretive guidelines for agents reported without interpretations.

 

For information regarding CLSI and EUCAST susceptibility interpretations, see Susceptibility Interpretative Category Definitions.

Day(s) Performed

Monday through Sunday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87081-Helicobacter pylori culture

87077-Bacteria identification (if appropriate)

87153-Aerobe Ident by Sequencing (if appropriate)

87176-Tissue processing (if appropriate)

87181-Susceptibility per drug and per organism for drugs not in routine battery (if appropriate)

87186-Antimicrobial Susceptibility, Aerobic Bacteria, MIC-per organism for routine battery (if appropriate)

87150-H pylori + Clarithro Resistance PCR (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HELIS Helicobacter pylori Culture + Susc 587-6

 

Result ID Test Result Name Result LOINC Value
HELIS Helicobacter pylori Culture + Susc 587-6

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
GID Bacteria Identification No, (Bill Only) No
ISAE Aerobe Ident by Sequencing No, (Bill Only) No
TISSR Tissue Processing No, (BIll Only) No
MIC Susceptibility, MIC No, (Bill Only) No
SUS Susceptibility No, (Bill Only) No
HPCR1 H pylori + Clarithro Resistance PCR No, (Bill Only) No

Testing Algorithm

When this test is ordered, the reflex tests may be performed at an additional charge.

 

When Helicobacter pylori is isolated, identification will be confirmed, and susceptibility testing performed. The routine susceptibility panel includes amoxicillin, clarithromycin, levofloxacin, metronidazole, rifampin, and tetracycline.

 

If an isolate of H pylori does not grow for susceptibility testing, additional testing for H pylori with clarithromycin resistance prediction may be performed.

Report Available

7 to 28 days

NY State Approved

Yes

Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-Microbiology Test Request (T244)

-Gastroenterology and Hepatology Test Request (T728)