Test ID FFES Estradiol Free, Serum (includes Estradiol and SHBG)
Method Name
Liquid chromatography tandem mass spectrometry (LC/MS/MS); equilibrium dialysis; immunoradiometric assay (IRMA)
Reporting Name
Free Estradiol with SHBGSpecimen Type
Serum RedSpecimen Required
Draw blood in a plain red-top tube (serum gel tube is acceptable). Spin down, pour off into plastic vial within 45 minutes of collection, and send 3 mL serum frozen.
Specimen Minimum Volume
1.0 mL Note: This volume does not allow for repeat testing.
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum Red | Frozen (preferred) | 180 days | |
Ambient | 48 hours | ||
Refrigerated | 48 hours |
Reference Values
Estradiol, Serum MS
Units: pg/mL
Age Range
Newborn
Levels are markedly elevated at birth and fall rapidly during the first week to prepubertal values of <15.
Males <6 m
Levels increase to 10 - 32 between 30 and 60 days, then decline to prepubertal levels of <15 by six months.
Females <1 y
Levels increase to 5.0 - 50 between 30 and 60 days, then decline to prepubertal levels of <15 during the first year.
Prepubertal <15
Adult Males 8.0 - 35
Adult Females
Follicular 30 - 100
Luteal 70 - 300
Postmenopausal <15
Free Estradiol, Percent
Units: %
Age Range
Adult Males 1.7 - 5.4
Adult Females 1.6 - 3.6
Free Estradiol, Serum
Units: pg/mL
Age Range
Adult Males 0.2 - 1.5
Adult Females 0.6 - 7.1
Sex Hormone Binding Globulin
Units: nmol/L
Age Range
Infants (1 - 23m) 60.0 - 252.0
Prepubertal 72.0 - 220.0
Pubertal
Males 16.0 - 100.0
Females 36.0 - 125.0
Adult Males
20 - 49 y 16.5 - 55.9
>49y 19.3 - 76.4
Adult Females
20 – 49y 24.6 - 122.0
>49y 17.3 - 125.0
Day(s) Performed
Varies
Performing Laboratory
Esoterix EndocrinologyCPT Code Information
82681 – Free Estradiol
84270 - Sex Hormone binding globulin
82670 - Estradiol
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
FFES | Free Estradiol with SHBG | Not Provided |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
Z0259 | Estradiol, Serum | 35384-7 |
Z0228 | Free Estradiol, Percent | 14960-9 |
Z0229 | Free Estradiol, Serum | 2240-0 |
Z0230 | Sex Hormone Binding Globulin (SHBG) | 13967-5 |