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HelpTest ID ESTS Estradiol, Rapid, Immunoassay, Serum
Useful For
Rapid assessment of ovarian status, including follicle development, for assisted reproduction protocols (eg, in vitro fertilization)
Establishing time of ovulation and optimal time for conception
Method Name
Electrochemiluminescent Immunoassay (ECLIA)
Reporting Name
Estradiol Rapid, Immunoassay, SSpecimen Type
SerumOrdering Guidance
This assay is for reproductive assessment (eg, IVF, conception). For other clinical indications, order EEST / Estradiol, Serum.
The preferred method for measurement of low serum estradiol concentrations in children, males, and postmenopausal females is liquid chromatography-tandem mass spectrometry (LC-MS/MS), order EEST / Estradiol, Serum.
This test should not be used when monitoring estradiol concentrations in patients being treated with Fulvestrant due to possible cross-reactivity. In these patients, estradiol concentrations should be measured using mass spectrometry; order EEST / Estradiol, Serum.
Specimen Required
Patient Preparation: For 12 hours before specimen collection, patient should not take multivitamins or dietary supplements (eg, hair, skin, and nail supplements) containing biotin (vitamin B7).
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Within 2 hours of collection, centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.4 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Frozen (preferred) | 180 days | |
| Refrigerated | 5 days | ||
| Ambient | 24 hours |
Reference Values
Males: 10-40 pg/mL
Females
Premenopausal: 15-350 pg/mL*
Postmenopausal: <10 pg/mL
*Estradiol concentrations vary widely throughout the menstrual cycle
Day(s) Performed
Monday through Sunday
Report Available
Same day/1 to 2 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
82670
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| ESTS | Estradiol Rapid, Immunoassay, S | 2243-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| ESTS | Estradiol Rapid, Immunoassay, S | 2243-4 |