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HelpTest ID COCOU Cortisol/Cortisone, Free, 24 Hour, Urine
Useful For
Screening test for Cushing syndrome (hypercortisolism)
Assisting in diagnosing acquired or inherited abnormalities of 11-beta-hydroxy steroid dehydrogenase (cortisol to cortisone ratio)
Diagnosis of pseudo-hyperaldosteronism due to excessive licorice consumption
This test has limited usefulness in the evaluation of adrenal insufficiency.
Special Instructions
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
Cortisol/Cortisone, Free, USpecimen Type
UrineNecessary Information
24-Hour volume (in milliliters) is required.
Specimen Required
Supplies: Urine Tubes, 10-mL (T068)
Submission Container/Tube: Plastic, urine tube (T068)
Specimen Volume: 5 mL
Collection Instructions:
1. Add 10 g of boric acid as preservative at start of collection.
2. Collect urine for a full 24 hours (required) and record the total volume.
Additional Information: See Urine Preservatives-Collection and Transportation for 24-Hour Urine Specimens for multiple collections.
Specimen Minimum Volume
3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Urine | Refrigerated (preferred) | 14 days | |
| Frozen | 28 days | ||
| Ambient | 72 hours |
Reference Values
CORTISOL
0-2 years: not established
3-8 years: 1.4-20 mcg/24 h
9-12 years: 2.6-37 mcg/24 h
13-17 years: 4.0-56 mcg/24 h
≥18 years: 3.5-45 mcg/24 h
CORTISONE
0-2 years: not established
3-8 years: 5.5-41 mcg/24 h
9-12 years: 9.9-73 mcg/24 h
13-17 years: 15-108 mcg/24 h
≥18 years: 17-129 mcg/24 h
Use the factors below to convert each analyte from mcg/24 h to nmol/24 h:
Conversion factors
Cortisol: mcg/24 hours x 2.76=nmol/24 h (molecular weight=362.5)
Cortisone: mcg/24 hours x 2.78=nmol/24 h (molecular weight=360)
For International System of Units (SI) conversion for Reference Values, see www.mayocliniclabs.com/order-tests/si-unit-conversion.html
Day(s) Performed
Monday through Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
82530-Cortisol; free
82542
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| COCOU | Cortisol/Cortisone, Free, U | 101319-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 8546 | Cortisol, U | 14158-0 |
| 10327 | Cortisone, U | 14044-2 |
| TM93 | Collection Duration (h) | 13362-9 |
| VL47 | Volume (mL) | 3167-4 |