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HelpTest ID CMVC8 Cytomegalovirus (CMV) CD8 T-Cell Immune Competence, Quantitative Assessment by Flow Cytometry, Blood
Test Down Notes
This test is temporarily unavailable due to reagent issues. For additional details, see test update here.
Useful For
Assessing cytomegalovirus (CMV)-specific immune competence in allo-hematopoietic stem cell transplantation patients who are at risk for developing late CMV disease (beyond day 100 after transplant)
Assessing CMV-specific immune competence in solid organ transplant patients who are at high risk for CMV reactivation posttransplant
Monitoring immune competence in patients post-primary CMV infection after transplant who are at risk for CMV reactivation after the cessation of antiviral prophylaxis
Identifying individuals who are likely to be protected from posttransplant CMV infection and those who are at higher risk of CMV reactivation
The global CD8 T cell immune competence assay is useful for determining over immunosuppression within the CD8 T cell compartment, when used on transplant recipients and patients with autoimmune disorders receiving therapy with immunosuppressant agents
Special Instructions
Method Name
Flow Cytometry
Reporting Name
CMV CD8 T-Cell QN by Flow CytometrySpecimen Type
WB Sodium HeparinOrdering Guidance
Patient must be cytomegalovirus seropositive and have 1 or more of the 5 major histocompatibility complex alleles: HLA A1, A2, B7, B8, or B35 to utilize this assay.
Additional Testing Requirements
It is important to ascertain the patient's and the donor's cytomegalovirus serostatus, as well as the patient's major histocompatibility complex class I HLA haplotype, before ordering this assay; see 1DIS / Human Leukocyte Antigens (HLA) A-B-C Disease Association Typing Low Resolution, Blood.
Shipping Instructions
Testing performed Monday through Friday. Specimens not received by 4pm (CST) on Friday may be cancelled.
Collect and package specimen as close to shipping time as possible. Ship specimen overnight in an Ambient Shipping Box-Critical Specimens Only (T668) following the instructions in the box. It is recommended that specimens arrive within 24 hours of collection.
Samples arriving on the weekend and observed holidays may be canceled.
Necessary Information
Ordering healthcare professional name and phone number are required.
Specimen Required
Supplies: Ambient Shipping Box-Critical Specimens Only (T668)
Container/Tube: Green top (sodium heparin)
Specimen Volume: 20 mL
Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.
Additional Information: For serial monitoring, it is recommended that specimens are collected at the same time of day.
Specimen Minimum Volume
10 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| WB Sodium Heparin | Ambient | 48 hours | GREEN TOP/HEP |
Reference Values
Total CD3 T cells: 884-5830 x 10(3)/mL
Total CD8 T cells: 168-1847 x 10(3)/mL
Total CMV CD8 T cells: 0-115 x 10(3)/mL
The adult reference values were determined for healthy adult controls ages 20 to 80 years (n=94), for HLA A1, A2, B7, B8, and B35 alleles.
Reference values for cytomegalovirus (CMV) specific T cells that are functional (interferon-gamma+, IFN-g+) and have cytotoxic activity (CD107a and CD107b expression, CD107 a/b+):
Total CMV CD8 T-cells IFN-g: 0.028-52.200 x 10(3)/mL
Total CMV CD8 T-cells CD107a/b: 0.252-50.760 x 10(3)/mL
The 95% confidence interval reference values were determined from 102 healthy adult donors:
Interferon-gamma (IFN-gamma) expression (as % CD8 T cells): 10.3-56.0%
CD107a/b expression (as % CD8 T cells): 8.5-49.1%
The reference values were developed for each of the following 4 major histocompatibility complex class I alleles: A1, A2, B7, and B8 (n=45). We were unable to develop ranges for the B35 allele due to a lack of matching donors. The data is expressed as the absolute number of CMV-specific CD8 T cells that are IFN-gamma+ or CD107a/b+.
Day(s) Performed
Monday through Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86356 x 6
86359
86352
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| CMVC8 | CMV CD8 T-Cell QN by Flow Cytometry | 95184-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 28143 | Total CD3 T cells | 8122-4 |
| 28144 | Total CD8 T cells | 14135-8 |
| 28145 | CMV CD8 T cells A1 | 95185-5 |
| 28146 | CMV CD8 T cells A2 | 95202-8 |
| 28147 | CMV CD8 T cells B7 | 95201-0 |
| 28148 | CMV CD8 T cells B8 | 95200-2 |
| 28149 | CMV CD8 T cells B35 | 95199-6 |
| 28150 | Total CMV CD8 T cells | 95198-8 |
| 28151 | CMV CD8 T cells IFN-g A1 | 95197-0 |
| 28152 | CMV CD8 T cells IFN-g A2 | 95196-2 |
| 28153 | CMV CD8 T cells IFN-g B7 | 95195-4 |
| 28154 | CMV CD8 T cells IFN-g B8 | 95194-7 |
| 28155 | CMV CD8 T cells IFN-g B35 | 95193-9 |
| 28156 | Total CMV CD8 T cells IFN-g | 95192-1 |
| 28157 | CMV CD8 T cells CD107 A1 | 95191-3 |
| 28158 | CMV CD8 T cells CD107 A2 | 95190-5 |
| 28159 | CMV CD8 T cells CD107 B7 | 95189-7 |
| 28160 | CMV CD8 T cells CD107 B8 | 95188-9 |
| 28161 | CMV CD8 T cells CD107 B35 | 95187-1 |
| 28162 | Total CMV CD8 T cells CD107a/b | 95186-3 |
| 30693 | IFN-gamma expression | 95204-4 |
| 30694 | CD107a/b expression | 95203-6 |
| 28163 | Interpretation | 69052-9 |