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HelpTest ID CEE52 CD52 Cell Expression Evaluation, Varies
Ordering Guidance
This test should not be used as a shortened diagnostic panel. For a complete diagnostic B-cell, T-cell, or acute immunophenotyping panel, order LCMS / Leukemia/Lymphoma Immunophenotyping, Flow Cytometry, Varies.
This test evaluates CD52 expression only. For CD20 expression, order CEE20 / CD20 Cell Expression Evaluation, Varies. For CD49d expression, order CEE49 / CD49d Cell Expression Evaluation, Varies.
Shipping Instructions
Specimen must arrive within 4 days of collection.
Necessary Information
The following information is required:
1. The therapeutic monoclonal antibody being used or considered
2. The pertinent hematologic diseases that have been diagnosed or considered
3. Diagnostic immunophenotype of the neoplastic cells (when available, a pathology and/or flow cytometry report should be included).
4. Specimen source
5. Date and time of collection
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Whole Blood
Container/Tube:
Preferred: Yellow top (ACD solution A or B)
Acceptable: Lavender top (EDTA) or Green top (sodium heparin)Â
Specimen Volume: 10 mL
Collection Instructions:
1. Send specimen in original tube. Do not aliquot.
2. Label specimen as blood.
Specimen Stability Information: Ambient/Refrigerated ≤4 days
Specimen Type: Bone marrow
Container/Tube:
Preferred: Yellow top (ACD solution A or B)
Acceptable: Lavender top (EDTA) or Green top (sodium heparin) Specimen Volume: 1-5 mL
Collection Instructions:
1. Submission of bilateral specimens is not required.
2. Label specimen as bone marrow.
3. Send bone marrow specimen in original tube. Do not aliquot.
Specimen Stability Information: Ambient/Refrigerated ≤4 days
Forms
If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.
Useful For
Detecting cell-surface antigens on malignant cells that are potential therapeutic antibody targets, specifically CD52
Determining the eligibility of patients for monoclonal antibody therapies
Monitoring response to the therapeutic antibody
Testing Algorithm
A complete diagnostic B-cell, T-cell, or acute immunophenotyping panel is not performed. In some cases, a limited morphologic evaluation will be performed.
Method Name
Immunophenotyping
Reporting Name
CD52 Cell Expression Evaluation, VSpecimen Type
VariesSpecimen Minimum Volume
Blood: 3 mL
Bone Marrow Aspirate: 1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Varies | 4 days |
Reference Values
Normal individuals have B lymphocytes, T lymphocytes, or myeloid cells that express the corresponding cell-surface antigens in question.
Day(s) Performed
Monday through Saturday
Report Available
1 to 4 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
88184-Flow cytometry; first cell surface, cytoplasmic or nuclear marker
88185 x 3-Flow cytometry; additional cell surface, cytoplasmic or nuclear marker
88187-Flow Cytometry Interpretation, 2 to 8 Markers
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| CEE52 | CD52 Cell Expression Evaluation, V | 100991-9 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| CK166 | CEE52 Result | No LOINC Needed |
| CK167 | Final Diagnosis | 22637-3 |