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HelpTest ID CDGF Celiac Disease Gluten-Free Cascade, Serum and Whole Blood
Useful For
Evaluating patients suspected of having celiac disease who are currently (or were recently) on a gluten-free diet
Special Instructions
Method Name
Polymerase Chain Reaction (PCR)/Sequence-Specific Oligonucleotide Probe (SSO)
Reporting Name
Celiac Disease Gluten-Free CascadeSpecimen Type
SerumWhole Blood ACD-B
Ordering Guidance
This cascade should not be used in patients for whom human leukocyte antigen (HLA) DQ2/DQ8 typing has already been performed. For individuals who are positive for either DQ2 and/or DQ8, CDSP / Celiac Disease Serology Cascade, Serum should be ordered to assess for the presence of autoantibodies associated with celiac disease. For individuals who are negative for DQ2 and DQ8, no further testing is necessary as a diagnosis of celiac disease is unlikely.
Cascade testing is recommended for celiac disease. Cascade testing ensures that testing proceeds in an algorithmic fashion. The following cascades are available; select the appropriate one for your specific patient situation.
-CDCOM / Celiac Disease Comprehensive Cascade, Serum and Whole Blood: Complete testing including HLA DQ
-CDSP / Celiac Disease Serology Cascade, Serum: Complete serology testing excluding HLA DQ
-CDGF / Celiac Disease Gluten-Free Cascade, Serum and Whole Blood: For patients already adhering to a gluten-free diet
To order individual tests, see Celiac Disease Diagnostic Testing Algorithm
Specimen Required
Both whole blood and serum are required.
Specimen Type: Whole Blood
Container/Tube: Yellow top (ACD solution A or B)
Specimen Volume: 6 mL
Collection Instructions: Send whole blood in original tube. Do not aliquot.
Specimen Type: Serum
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 2 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial
Specimen Minimum Volume
Blood: 3 mL
Serum: 1.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 21 days | |
| Frozen | 21 days | ||
| Whole Blood ACD-B | Refrigerated (preferred) | ||
| Ambient | |||
Reference Values
HLA-DQ TYPING
Presence of HLA-DQ2 or HLA-DQ8 alleles associated with celiac disease
Day(s) Performed
Profile tests: Monday through Friday; Reflex tests: Monday through Saturday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
81376 x 2
82784 (if appropriate)
86258 (if appropriate)
86364 (if appropriate)
86231 (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| CDGF | Celiac Disease Gluten-Free Cascade | 94493-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| DQA | DQ alpha 1 | 94495-9 |
| 28991 | Celiac Disease Interpretation | 69048-7 |
| DQB | DQ beta 1 | 53938-7 |
| CELIG | Celiac gene pairs present? | 48767-8 |
Test Classification
See Individual Test IDsProfile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| CELI2 | HLA-DQ Typing | Yes, (Order CELI) | Yes |
| CDGF1 | Celiac Disease Interpretation | No | Yes |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| TTGA | Tissue Transglutaminase Ab, IgA, S | Yes | No |
| DAGL | Gliadin(Deamidated) Ab, IgA, S | Yes | No |
| DGGL | Gliadin(Deamidated) Ab, IgG, S | Yes | No |
| TTGG | Tissue Transglutaminase Ab, IgG, S | Yes | No |
| IGA | Immunoglobulin A (IgA), S | Yes | No |
Testing Algorithm
If HLA-DQ typing is positive or equivocal for DQ2 or DQ8, then IgA, tissue transglutaminase IgA and IgG, and deamidated gliadin IgA and IgG antibody testing will be performed at an additional charge.
The following algorithms are available:
Report Available
9 to 11 daysNY State Approved
YesForms
If not ordering electronically, complete, print, and send Gastroenterology and Hepatology Test Request (T728) with the specimen