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HelpTest ID BRAFB Cell-Free DNA BRAF V600, Blood
Useful For
An alternative to invasive tissue biopsies for the determination of BRAF V600E and V600K alterations
Identification of patients with cancer who are most likely to benefit from targeted therapies
This test is not intended for serial monitoring of patients with cancer or as a screening test to identify cancer.
Genetics Test Information
This test evaluates cell-free DNA (cfDNA) in the peripheral blood for the presence of BRAF V600E or V600K alterations in patients with cancer and can be used to determine if these patients are candidates for targeted therapies.
Highlights
This test evaluates peripheral blood for BRAF alterations in cell-free DNA.
Detection of BRAF alterations in melanoma patients can be used as an alternative for BRAF analysis of tissue.
Current data suggests that the efficacy of BRAF-targeted therapy and anti-MEK therapy in melanoma is limited to patients whose tumors harbor a V600E or V600K alteration.
Method Name
Digital Droplet Polymerase Chain Reaction (ddPCR)
Reporting Name
cfDNA BRAF V600 Test, BloodSpecimen Type
Whole bloodOrdering Guidance
This test is not a prenatal screening test.
Shipping Instructions
1. Samples should be transported at ambient temperature or refrigerated (4° C)
2. Samples are viable for 7 days in the Streck Black/Tan Top Tube Kit (T715)
Specimen Required
Supplies: Streck Black/Tan Top Tube Kit (T715)
Container/Tube: Streck Cell-Free DNA blood collection kit
Specimen Volume: Two 10-mL Streck Cell-Free DNA blood collection tubes
Additional Information: Only blood collected in Streck Cell-Free DNA tubes will be accepted for analysis. Whole blood will be processed to produce platelet-poor plasma before cfDNA isolation.
Specimen Minimum Volume
One 10 mL Streck cell-free DNA blood collection tube
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole blood | Ambient (preferred) | 7 days | |
| Refrigerated | 7 days |
Day(s) Performed
Varies
Report Available
5 to 10 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
81210
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| BRAFB | cfDNA BRAF V600 Test, Blood | 93690-6 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 48044 | Result Summary | 50397-9 |
| 48045 | Result | 93690-6 |
| 48046 | Interpretation | 69047-9 |
| 48047 | Additional Information | 48767-8 |
| 48048 | Specimen | 31208-2 |
| 48049 | Source | 31208-2 |
| 48050 | Released By | 18771-6 |
| 606201 | Method | 85069-3 |
| 606202 | Disclaimer | 62364-5 |
NY State Approved
YesForms
If not ordering electronically, complete, print, and send an Oncology Test Request (T729) with the specimen.
Reference Values
An interpretive report will be provided.