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Test ID BIOTN Biotin, Serum


Ordering Guidance


If testing for biotinidase deficiency is requested, order BIOTS / Biotinidase, Serum.



Shipping Instructions


Freeze specimens within 3 days of collection and send frozen.



Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Useful For

Measurement of biotin in serum

 

Assessment of biotin concentrations in individuals taking biotin supplements

 

Investigation of unexpected results from immunoassays that utilize biotin-streptavidin detection methods

 

This test is not useful as a screen for biotinidase deficiency.

Highlights

Serum biotin is usually at relatively low endogenous concentrations but can be found at high concentrations if vitamin and herbal supplements containing biotin are taken.

 

This test measures free biotin concentrations in serum and can be used to determine if a patient's high biotin concentrations are likely from biotin supplementation or treatment.

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Biotin, S

Specimen Type

Serum

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Frozen (preferred) 30 days
  Refrigerated  7 days
  Ambient  24 hours

Reference Values

≥18 years: ≤0.3 ng/mL

Reference values have not been established for patients who are <18 years of age.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

84591

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BIOTN Biotin, S 1980-2

 

Result ID Test Result Name Result LOINC Value
606867 Biotin, S 1980-2

NY State Approved

Yes

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

Day(s) Performed

Tuesday

Report Available

2 to 7 days